Expression of telomerase reverse transcriptase (TERT) in malignant mesotheliomas
Identifieur interne : 00B729 ( Main/Exploration ); précédent : 00B728; suivant : 00B730Expression of telomerase reverse transcriptase (TERT) in malignant mesotheliomas
Auteurs : Fumiyuki Kumaki [États-Unis] ; Toshiaki Kawai [Japon] ; Andrew Churg [Canada] ; Francoise B. Galateau-Salle [France] ; Philip Hasleton [Royaume-Uni] ; Douglas Henderson [Australie] ; Victor Roggli [États-Unis] ; William D. Travis [États-Unis] ; Philip T. Cagle [États-Unis] ; Victor J. Ferrans [États-Unis]Source :
- The American journal of surgical pathology [ 0147-5185 ] ; 2002.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
To evaluate the usefulness of determinations of telomerase activity for distinguishing malignant from benign mesothelial lesions, immunohistochemical (using a rabbit polyclonal antibody and the peroxidase method; n = 68) and in situ hybridization (using sense and antisense oligonucleotide probes; n = 46) studies were made on malignant mesotheliomas (epithelioid, 39; sarcomatoid, 18, including 2 of the desmoplastic type; and biphasic, 11) and 19 benign mesothelial lesions (benign mesothelial hyperplasia, 3; and reactive pleuritis, 16). In addition, biochemical studies of telomerase activity were made in 9 of the malignant mesotheliomas. Telomerase activity was detected histochemically in all but one of the malignant mesotheliomas, but only in one (pleuritis) of the benign lesions, in which it was present only in activated lymphocytes. Antisense hybridization signals indicated the presence of telomerase mRNA mainly in the cytoplasm of the malignant cells. Sense probes gave negative results. Biochemical determinations revealed a strong telomerase activity in the 9 malignant mesotheliomas examined. This study demonstrates the usefulness of immunohistochemical staining for the evaluation of mesotheliomas. The required immunostaining can be performed using paraffin sections of formalin-fixed tissues.
Affiliations:
- Australie, Canada, France, Japon, Royaume-Uni, États-Unis
- Angleterre, Caroline du Nord, Grand Manchester, Maryland, Texas
- Manchester
- Université de Manchester
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Travis</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Armed Forces Institute of Pathology</s1><s2>Washington, DC</s2><s3>USA</s3><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Armed Forces Institute of Pathology</wicri:noRegion></affiliation></author><author><name sortKey="Cagle, Philip T" sort="Cagle, Philip T" uniqKey="Cagle P" first="Philip T." last="Cagle">Philip T. Cagle</name><affiliation wicri:level="2"><inist:fA14 i1="09"><s1>Baylor College of Medicine</s1><s2>Houston, Texas</s2><s3>USA</s3><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Ferrans, Victor J" sort="Ferrans, Victor J" uniqKey="Ferrans V" first="Victor J." last="Ferrans">Victor J. Ferrans</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Pathology Section, National Heart, Lung and Blood Institute, National Institutes of Health</s1><s2>Bethesda, Maryland</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j" type="main">The American journal of surgical pathology</title><title level="j" type="abbreviated">Am. j. surg. pathol.</title><idno type="ISSN">0147-5185</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The American journal of surgical pathology</title><title level="j" type="abbreviated">Am. j. surg. pathol.</title><idno type="ISSN">0147-5185</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biochemical analysis</term><term>Differential diagnostic</term><term>Double labelling</term><term>Human</term><term>Hybridization</term><term>Hyperplasia</term><term>Immunofluorescence</term><term>Immunohistochemistry</term><term>In situ</term><term>Malignant mesothelioma</term><term>Mesothelium</term><term>Pleura</term><term>Pleurisy</term><term>RNA-directed DNA polymerase</term><term>Telomerase</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Mésothéliome malin</term><term>Plèvre</term><term>Hyperplasie</term><term>Pleurésie</term><term>Mésothélium</term><term>Immunohistochimie</term><term>Immunofluorescence</term><term>Marquage double</term><term>Hybridation</term><term>In situ</term><term>Analyse biochimique</term><term>Telomerase</term><term>RNA-directed DNA polymerase</term><term>Diagnostic différentiel</term><term>Homme</term><term>Pleurite</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To evaluate the usefulness of determinations of telomerase activity for distinguishing malignant from benign mesothelial lesions, immunohistochemical (using a rabbit polyclonal antibody and the peroxidase method; n = 68) and in situ hybridization (using sense and antisense oligonucleotide probes; n = 46) studies were made on malignant mesotheliomas (epithelioid, 39; sarcomatoid, 18, including 2 of the desmoplastic type; and biphasic, 11) and 19 benign mesothelial lesions (benign mesothelial hyperplasia, 3; and reactive pleuritis, 16). In addition, biochemical studies of telomerase activity were made in 9 of the malignant mesotheliomas. Telomerase activity was detected histochemically in all but one of the malignant mesotheliomas, but only in one (pleuritis) of the benign lesions, in which it was present only in activated lymphocytes. Antisense hybridization signals indicated the presence of telomerase mRNA mainly in the cytoplasm of the malignant cells. Sense probes gave negative results. Biochemical determinations revealed a strong telomerase activity in the 9 malignant mesotheliomas examined. This study demonstrates the usefulness of immunohistochemical staining for the evaluation of mesotheliomas. The required immunostaining can be performed using paraffin sections of formalin-fixed tissues.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Canada</li><li>France</li><li>Japon</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Caroline du Nord</li><li>Grand Manchester</li><li>Maryland</li><li>Texas</li></region><settlement><li>Manchester</li></settlement><orgName><li>Université de Manchester</li></orgName></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Kumaki, Fumiyuki" sort="Kumaki, Fumiyuki" uniqKey="Kumaki F" first="Fumiyuki" last="Kumaki">Fumiyuki Kumaki</name></region><name sortKey="Cagle, Philip T" sort="Cagle, Philip T" uniqKey="Cagle P" first="Philip T." last="Cagle">Philip T. Cagle</name><name sortKey="Ferrans, Victor J" sort="Ferrans, Victor J" uniqKey="Ferrans V" first="Victor J." last="Ferrans">Victor J. Ferrans</name><name sortKey="Roggli, Victor" sort="Roggli, Victor" uniqKey="Roggli V" first="Victor" last="Roggli">Victor Roggli</name><name sortKey="Travis, William D" sort="Travis, William D" uniqKey="Travis W" first="William D." last="Travis">William D. Travis</name></country><country name="Japon"><noRegion><name sortKey="Kawai, Toshiaki" sort="Kawai, Toshiaki" uniqKey="Kawai T" first="Toshiaki" last="Kawai">Toshiaki Kawai</name></noRegion></country><country name="Canada"><noRegion><name sortKey="Churg, Andrew" sort="Churg, Andrew" uniqKey="Churg A" first="Andrew" last="Churg">Andrew Churg</name></noRegion></country><country name="France"><noRegion><name sortKey="Galateau Salle, Francoise B" sort="Galateau Salle, Francoise B" uniqKey="Galateau Salle F" first="Francoise B." last="Galateau-Salle">Francoise B. Galateau-Salle</name></noRegion></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Hasleton, Philip" sort="Hasleton, Philip" uniqKey="Hasleton P" first="Philip" last="Hasleton">Philip Hasleton</name></region></country><country name="Australie"><noRegion><name sortKey="Henderson, Douglas" sort="Henderson, Douglas" uniqKey="Henderson D" first="Douglas" last="Henderson">Douglas Henderson</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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